Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Cavicide (Warehouse, Cat # 79300360) 6. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Our absorbance plate readers feature our PathCheck Sensor. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Last lab had ours for years and never had a problem with it. Choose cables that meet the requirement described in this appendix. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. Optimal instrument settings are the same for all three readers. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. The user manuals cover installation, troubleshooting, and validation of the SpectraMax M5 and its accessories. 6 lbs. BRET does not work on the Spectramax M5 due to a sensitivity issue and to the fact that the BRET assay has very weak signal in general. Flexible for 6-well to 384-well microplates. Here is a Molecular Devices Spectramax M5 Microplate Reader for sale. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. View the Molecular Devices websiteResults by SpectraMax M5 from multiple independent donors are given. 4. . The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Weight. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. S. Fix: While proper use of the Shake button is to hold the button down for the duration of the desired mixing time, repeated sequential clicking is now allowed. Choose from a three- (M3), four- (M4), or SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 26 0112-0115J ApplicationsofAbsorbance Absorbance-baseddetectioniscommonlyusedtoevaluatechangesincolororturbidity,. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. SpectraMax fluorescence microplate reader with SoftMax® Pro Software, is a quick, sensitive detection method for proteins. . com A. . 40266 Email: drugdiscoverytech@lifetech. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. Molecular Devices Spectramax M5 Plate Reader is available from The Lab World Group. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. In addition, the SpectraMaxMolecular Devices. used. The time course of the cleavage reaction was monitored by loss of FRET (excitation, 450 nm; emission, 655 nm) with 15 reads/well and 4–5-s read intervals on a SpectraMax M5 plate reader (Molecular Devices). The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. edu) to be added as a trained user in iLab. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. Molecular Devices ®(MD) SpectraMax 340PC reader MD VersaMax™ Tunable Microplate readers MD SpectraMax® L Microplate Luminometers MD SpectraMax® M5 systems MD ®SpectraMax 96/384-well plate reader Gemini fluorescence plate reader Automation Hamilton Microlab® STAR™ liquid handling systemThe SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. 1). 1. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. View the Molecular Devices websiteResults by SpectraMax M5 from multiple independent donors are given. Molecular Devices Spectramax M5 luminometer: Krishnamurthy lab-Praveen (lab WO3213, x4605) Spectramax plate reader: yes Beckman LS6000IC Scintillation counter: Bill Taylor (office WO4262B, x1966) WO3242 liquid scintillation counter Department backup -80oC freezer: Song-Tao Liu (WO4254, x7857) Facilities Facility: Department. Time-Resolved Fluorescence (TRF) Fluorescence Polarization (FP) Glow Luminescence (Lumi) The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing. view more . Published Dec 16, 2020 | Updated Dec 16, 2020. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. 306 plate air cal. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 2 SpectraMax MiniMax300ImagingCytometer(cytometer) 3. The following document is intended to demonstrate setup of this. no. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. Fluorescence spectra of CDs were measured using a microplate reader (SpectraMax M5, Molecular Devices). 5. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. Spectramax M5 plate reader (96/384 format) Multimode photometrics for endpoint or kinetic measurements. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. The substrate solution (100 μL/well) was subsequently employed for 5-min reaction and the absorbance at 370 nm was monitored on a SpectraMax M5 plate reader (Molecular Devices). The Spectramax performs spectral scanning in 1 nm increments, and up to six wavelengths. STAKMAX. . Detection modalities include absorbance (UV-Vis), fluorescence intensity, fluorescence polarization, time-resolved fluorescence and luminescence. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Table of Contents. Wavelength Range. The following document is intended to demonstrate setup of this instrument. SoftMax Pro software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Devices SpectraMax i3, SpectraMax Paradigm, or SpectraMax M5 Multi-Mode Microplate Readers. The following document is intended to demonstrate setup of this instrument and provide representativeUsed together with the SpectraMax iD3 reader, the EarlyTox Cell Viability Assay Kits enable direct measurement of live and dead or apoptotic cells with a simple workflow and the increased throughput offered by a microplate format. We would like to show you a description here but the site won’t allow us. The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Call 516-939-0888 to check current. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. Sale. Pick the best speed, sensitivity, and price for your needs. . SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). The optical performance is comparable to a top-of-the-line dedicated The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. FlexStation® 3 Multi-Mode Microplate Reader. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. 0 out of 5 $ 17,999 $ 16,995. , ~488 nm), has the optical. SpectraMax M5e Multi-Mode Microplate Reader. SpectraMax M Series multi-mode microplates are configurable readers with triple-mode cuvette ports, validation tools, and IQ/OQ. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. . spr * SpectraMax i3(x) SpectraTest ABS2 v1. . The patented AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The day after, a final concentration of 1 μmol/L ARS1620, 20 nmol/L SMARTpool siRNA or the combination of the 2 was added in 5 replicates (wells). com A. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. Agilent VWorks Automation Control soft-ware manages all processes and incubation2 Luminescence integration time For supported SpectraMax M5 microplate readers (minimum firmware requirements apply), integration times of 3, 5, 7, and 10 seconds were added for luminescence read mode. Features. すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. SpectraMax M5 microplate reader. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax Microplate Stacker for walk-away processing. In this product video, see how the SpectraMax M5 quickly reads samples. 0 (GraphPad Software, La Jolla, CA, USA) and SPSS version 23. 5. SoftMax® Pro Microplate Data Acquisition and Analysis Software serves as the user interface to the microplate reader. Currently we have over 300 Windows 7/10 compatible instrument drivers to control equipment from 147 manufacturers, meaning that we are likely to have your instrument already in our library. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well Qualify the fluorescence performance of the SpectraMax . Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. . Softmax Pro GxP Software 6. The test solution was by diluting FCV to PBS/ Glycerol solutions (5 μM, 1. Step 4: Incubate at room temperature for 1 hour, protected from light. . Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. With its 5-color monochromator detection head, MOLECULAR DEVICES SpectraMax M5 is the ideal choice for high-throughput applications, providing superior results in the analysis of a. 19. The dual monochromator optics allow. viiProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3 reader What is the best way to use fluorescence (FL) mode with cuvettes? 1. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. . SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. Or, with the reader specifications noted from the Instrument Description section of your reader's manual, as well as those for your PC, you can calculate needs more closely. This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. This analysis indicated that, after 48 hours incubation, there was a significant difference (P < 0. D 1. It is available to all researchers. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. ability to detect 16 ng/well of DNA and to quantitate 50 ng of . 2. fluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter Created DateThe Spectramax M5 has similar detection capability to the Analyst GT and LMax II 384 (Figure 4), so we are confident that its performance is also similar when measuring the enzymes in cell lysates. together with SpectraMax, VersaMax, VMax, and EMax microplate readers from Molecular Devices, offer a quality assurance screening solution for the detection of melamine contamination in such food products as milk, infant formula, pet food, and confectionery. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The dual monochromator optics allow the widest range of applications to be utilized for bior. The . Add to Cart. Overall, SpectraMax M5 is a powerful lab tool that combines advanced detection capabilities, user-friendly software, and comprehensive accessory options. 2. For example, microplates containing different samples can be read using theThe interval between reads was set at 15 s or 60 s, respectively. 5. . Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. . Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini EM reader, SpectraMax M5/M5e reader, FlexStation 3 reader, SpectraMax M4 reader Which is better: top-read or bottom-read? You can use either. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. 315 can't find zero order. SpectraMax M5 Multi-Mode Microplate Reader. Molecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. 2 mm long, 86. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. the IMAP Platform and smoothly transition into high-throughput screening. 314 low lamp level warning. free shipping. The SpectraMax® M5 Multi-Mode Microplate Reader is ideally suited for assay development and high-throughput screening using IMAP assays. . Description 1. April 22 Asking Price: $15,990 USD Shoukat Mumtaz / Zantek Medical phone: +1 (952) 657-7977 (3). Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. 72 MB. These robust readers have been placed in labs from Antarctica to the. , ~488 nm), has the optical. Step 1: Open the SoftMax Pro IMAP FP protocol. The SpectraMax M5/M5e & FlexStation 3 instruments use SoftMaxPro® Data Acquisition & Analysis Software. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. . Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. e. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. Both full length human PI4KIIIB and the truncated PI4KIIIB crystal construct with the c-term were run at 200 nM. 2. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. 304 signal level saturation. In this. The absorbance of MPO was calculated using the SpectraMax M5 (Molecular Devices Corporation, San Jose, CA, USA) at 460 nm. SoftMax Pro Software combines reader control and data analysis and provides sophisticated curve fitting and statistics for more in-depth bioassay analysis. The SpectraMax® Gemini™ XPS Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. We would like to show you a description here but the site won’t allow us. . HEALTH AND SAFETY CONSIDERAIONSThe Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Small, unbound molecules rotate rapidly in solution (top), while large,. The following document is. When used with SoftMax® Pro Data Acquisition and Analysis Software, it can automatically normalize the well absorbance to a cuvette equivalentAnalytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Cables. Devices SpectraMax i3, SpectraMax Paradigm, or SpectraMax M5 Multi-Mode Microplate Readers. KeySpan adapter for SoftMax Pro and SpectraMax readers. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forF1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9THE SPECTRAMAX PLUS Two ways to correct pathlength The SPECTRAmax PLUS gives you two options when making pathlength-cor-rected measurements: 1) putting the aqueous solvent into a clean 1 cm reference cuvette in the instrument’s cuvette port and having the instrument base the calcu-lations on the A 1000 and A 900Molecular Devices社の製品: マイクロプレート リーダー用ソフト SoftMax® Proに関するすべての情報をご覧ください。価格、見積もり、お近くの販売店を知るにはメーカーまたは本社に直接お問い合わせくだ. Description. 6. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Molecular Devices SpectraMax M5 Multimode Microplate Reader. There were 12 replicate wells per dilution in both 96-well and 384- well plates. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices Supplier: Molecular Devices Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers single. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesand emission wavelengths for SpectraMax M5 fluorescence microplate readers are as follows: excitation at 490 nm and emission at 525 nm, with 515-nm emission cutoff filter (note that these are different from the wavelengths recommended for filter-based readers in the Quant-iT PicoGreen product insert). The hydrolysis was monitored continuously by top-read fluorescence using a Spectramax M5 multimode plate reader (Molecular Devices, USA) from the release of 7-amino-4-methylcoumarin (AMC) using an excitation wavelength of 380 nm and monitoring the. HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax M5, Analyst HT or Analyst GT. The following document is intended to demonstrate setup of this instrument. 312 gain calibration failed. . 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. . SpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. 1. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Absorbance (UV – VIS) Fluorescence; Glow luminescence; Time-resolved fluorescence; Fluorescent polarization; ABI Viia7 qPCR instrument (96 format) – SYBR/FAM/VIC/ROX calibrated. Molecular Devices SpectraMax 190 Microplate Absorbance Spectrophotometer. View the Molecular Devices websiteSpectraMax® M5 multimode microplate reader from Molecular Devices to measure both reporters in a completely homogeneous manner, in the same well of a 96-well plate, without cell lysis. Fluorescent Polarization: Solid black plates, as above. The following document is intended to demonstrate setup of this. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. edu) to be added as a trained user in iLab. It does not work as expected. HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The ability to measure inhibition of phosphorylation by Erlotinib was tested by incubating cells with increasing concentrations of Erlotinib, then stimulating cells with 0. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Lower limits of detection for ATP determined using the ATPlite . Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. +44-118-944-8000. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. free tracer decreases, resulting in an. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. Add to Cart. • Tel. Sale. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. When the correct COM port is selected, the. LUMINESCENCE The SpectraMax M2 and SpectraMax M2e are microplate spectrofluorometers with photomultiplier tube detection. Bookings are free, but you will need to use iLab to book. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. LMax, SpectraMax ®, VersaMax™, VMax , EMax , UVMax, FlexStation®, and ThermoMax benchtop Microplate Readers. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. NORTH AMERICA. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. Read fluorescence polarization at room temperature (RT, 19–25°C). Choose from a three- (M3), four- (M4), orSpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. All reagents were brought to room temperature before use. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. es • Flexibility for your assay needs: Robotics compatibility for increased throughput:In this application note, we describe how the SpectraMax® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. . Step 3. Table of Contents. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. In the M2, M5 and Plus 384, the light. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. The M5 can read volumes down to 2uL with the uMax Low Volume. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. The SpectraMax ABS Plus reader utilizes Molecular . The hydrolysis was monitored continuously by top-read fluorescence using a Spectramax M5 multimode plate reader (Molecular Devices, USA) from the release of 7-amino-4-methylcoumarin (AMC) using an excitation wavelength of 380 nm and monitoring the emission at 460 nm over 1 h. 1) EarlyTox NucView 488 Assay (6. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. SpectraMax Glo and DuoLuc. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Find many great new & used options and get the best deals for Molecular Devices SpectraMax M5 Microplate Reader with 90-Day Warranty at the best online prices at eBay! Free shipping for many products!Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 7. Molecular Devices Spectramax M5 luminometer: Krishnamurthy lab-Praveen (lab WO3213, x4605) Spectramax plate reader: yes Beckman LS6000IC Scintillation counter: Bill Taylor (office WO4262B, x1966) WO3242 liquid scintillation counter Department backup -80oC freezer: Song-Tao Liu (WO4254, x7857) Facilities Facility: Department. Spectral Range. The M5 can read volumes down to 2uL with the. Figure 1. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. In the M2, M5 and Plus. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuidePlace the plate into a SpectraMax M5 e plate reader (Molecular Devices). The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15. Wavelength Range. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 3. Description 1. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. you can use a resource. We would like to show you a description here but the site won’t allow us. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. 1. Typical LLDs were approximately 1–2 fg/well in the Analyst GT and 2–3 fg/well in the SpectraMax M5. SpectraMax®L Luminometer User Guide 0112-0174 B 11 The SpectraMax L Luminescence Microplate Reader can accommodate SBS-standard 96 and 384-well microplates with dimensions of 128. Introduction. ” SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. When the user applies a set temperature of 37°C, the top is heated to 37. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Five preferred detection modes. Lift up the lid over the chamber to insert or remove a cuvette. 2. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). . SpectraMax M3, M4, and M5/M5e readers. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. Turn on the microplate reader. Multimode Reader Comparison Chart. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. 190-1000. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic Gq-coupled receptor. Category 2 and 3 microplate readers use gradient heating as well. The assay applies fluorescence polarization to quantify Fc-containing IgG. The following document is intended to demonstrate setup of this instrument. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested. While they play an important role in generating data from a wide range of. +1 800-635-5577. Molecular Devices SpectraMax PLUS 384 Spectrophotometer System-New ABs1 Performance Testing August 2021. Plate chamber design of the SpectraMax category 1 microplate readers. Open the Softmax Pro software and select the FP option. Sensitive fluorescent quantitation of DNA with the Quant-iT. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Molecular Devices. 313 reference gain check fail. . 305 reference level saturation. 6: bioNova científica, s. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Softmax Pro GxP Software 6. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. . Also refer to this Multimode Reader Comparison Chart. SPECTRAmax GEMINI-XS spectrofluorometer was able to detect a specific GFP signal at all cell numbers as determined by One Way Analysis of Variance (ANOVA) (Table 1). Formula accessors for reporting o The accessor!sectionname will return the name of the SoftMax Pro section containing the. D 47 7. . HTRF-compatible SpectraMax Multi-Mode Readers. Page: 1/6. 3. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. . In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. Find regional support. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. 01) between the GFP-transfected and mock-transfected cells at an initial seeding. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. 0 out of 5 $ 17,999 $ 16,995. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. 9000-0756. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. Fluorescence Microplate Reader Comparison Chart.